{"id":6571,"date":"2024-10-31T14:03:41","date_gmt":"2024-10-31T07:03:41","guid":{"rendered":"https:\/\/biosciences.vn\/?post_type=product&#038;p=6571"},"modified":"2025-08-28T14:55:09","modified_gmt":"2025-08-28T07:55:09","slug":"nzy-m-mulv-first-strand-cdna-synthesis-kit-separate-oligos-2","status":"publish","type":"product","link":"https:\/\/biosciences.vn\/index.php\/product\/nzy-m-mulv-first-strand-cdna-synthesis-kit-separate-oligos-2\/","title":{"rendered":"NZY M-MuLV First-Strand cDNA\n  Synthesis Kit, separate oligos (MB17302)"},"content":{"rendered":"<p><span>B\u1ed9 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean NZY M-MuLV, oligo ri\u00eang bi\u1ec7t, l\u00e0 m\u1ed9t h\u1ec7 th\u1ed1ng bao g\u1ed3m t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n c\u1ea7n thi\u1ebft \u0111\u1ec3 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean, ngo\u1ea1i tr\u1eeb RNA khu\u00f4n m\u1eabu. C\u00e1c hexamer ng\u1eabu nhi\u00ean v\u00e0 c\u00e1c \u0111o\u1ea1n m\u1ed3i Oligo(dT)\u00a0<\/span><sub><span>18<\/span><\/sub><span>\u00a0\u0111\u01b0\u1ee3c cung c\u1ea5p trong c\u00e1c \u1ed1ng ri\u00eang bi\u1ec7t \u0111\u1ec3 thu\u1eadn ti\u1ec7n cho vi\u1ec7c l\u1ef1a ch\u1ecdn \u0111o\u1ea1n m\u1ed3i th\u00edch h\u1ee3p \u0111\u1ec3 b\u1eaft \u0111\u1ea7u ph\u1ea3n \u1ee9ng phi\u00ean m\u00e3 ng\u01b0\u1ee3c c\u1ee7a b\u1ea1n. cDNA s\u1ee3i \u0111\u01a1n thu \u0111\u01b0\u1ee3c ph\u00f9 h\u1ee3p \u0111\u1ec3 s\u1eed d\u1ee5ng trong PCR phi\u00ean m\u00e3 ng\u01b0\u1ee3c \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1eddi gian th\u1ef1c (RT-qPCR). B\u1ed9 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean NZY M-MuLV, oligo ri\u00eang bi\u1ec7t, \u0111\u01b0\u1ee3c b\u00e0o ch\u1ebf \u0111\u1ec3 cung c\u1ea5p s\u1ea3n l\u01b0\u1ee3ng cao c\u00e1c s\u1ea3n ph\u1ea9m cDNA to\u00e0n ph\u1ea7n v\u00e0 t\u0103ng \u0111\u1ed9 nh\u1ea1y trong RT-qPCR. Nguy\u00ean li\u1ec7u \u0111\u1ea7u v\u00e0o c\u00f3 th\u1ec3 dao \u0111\u1ed9ng t\u1eeb 10 pg \u0111\u1ebfn 5 \u00b5g RNA t\u1ed5ng s\u1ed1. B\u00ean c\u1ea1nh c\u00e1c hexamer ng\u1eabu nhi\u00ean v\u00e0 c\u00e1c \u0111o\u1ea1n m\u1ed3i Oligo(dT)\u00a0<\/span><sub><span>18<\/span><\/sub><span>\u00a0, b\u1ed9 s\u1ea3n ph\u1ea9m bao g\u1ed3m NZYRT 2\u00d7 Master Mix, kh\u00f4ng c\u00f3 oligo, ch\u1ee9a dNTP, MgCl2\u00a0<\/span><sub><span>v\u00e0<\/span><\/sub><span>\u00a0\u0111\u1ec7m RT \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a; \u0110\u1ec7m \u1ee7 10\u00d7 v\u00e0 h\u1ed7n h\u1ee3p enzyme RT NZYM-MuLV. H\u1ed7n h\u1ee3p enzyme RT NZYM-MuLV bao g\u1ed3m c\u1ea3 NZY M-MuLV Reverse Transcriptase (RNase H minus) v\u00e0 ch\u1ea5t \u1ee9c ch\u1ebf NZY Ribonuclease \u0111\u1ec3 b\u1ea3o v\u1ec7 RNA kh\u1ecfi s\u1ef1 ph\u00e2n h\u1ee7y do nhi\u1ec5m ribonuclease. RNase H (t\u1eeb\u00a0<\/span><i><span>E. coli<\/span><\/i><span>\u00a0) \u0111\u01b0\u1ee3c cung c\u1ea5p trong m\u1ed9t \u1ed1ng ri\u00eang \u0111\u1ec3 ph\u00e2n h\u1ee7y c\u1ee5 th\u1ec3 khu\u00f4n m\u1eabu RNA trong c\u00e1c lai cDNA-RNA sau khi t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean. Quy tr\u00ecnh n\u00e0y s\u1ebd c\u1ea3i thi\u1ec7n \u0111\u1ed9 nh\u1ea1y c\u1ee7a c\u00e1c ph\u1ea3n \u1ee9ng RT-qPCR ti\u1ebfp theo v\u00ec c\u00e1c \u0111o\u1ea1n m\u1ed3i PCR s\u1ebd li\u00ean k\u1ebft d\u1ec5 d\u00e0ng h\u01a1n v\u1edbi cDNA.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>B\u1ed9 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean NZY M-MuLV, oligo ri\u00eang bi\u1ec7t, l\u00e0 m\u1ed9t h\u1ec7 th\u1ed1ng bao g\u1ed3m t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n c\u1ea7n thi\u1ebft \u0111\u1ec3 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean, ngo\u1ea1i tr\u1eeb RNA khu\u00f4n m\u1eabu. C\u00e1c hexamer ng\u1eabu nhi\u00ean v\u00e0 c\u00e1c \u0111o\u1ea1n m\u1ed3i Oligo(dT)\u00a018\u00a0\u0111\u01b0\u1ee3c cung c\u1ea5p trong c\u00e1c \u1ed1ng ri\u00eang bi\u1ec7t \u0111\u1ec3 thu\u1eadn [&hellip;]<\/p>\n","protected":false},"featured_media":6394,"comment_status":"open","ping_status":"closed","template":"","meta":[],"pwb-brand":[933],"product_cat":[434,731,820],"product_tag":[],"class_list":{"0":"post-6571","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"pwb-brand-nzytech","7":"product_cat-khoa-hoc-su-song","8":"product_cat-sinh-hoc-phan-tu","9":"product_cat-rna-cdna-vi","11":"first","12":"onbackorder","13":"shipping-taxable","14":"purchasable","15":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>NZY M-MuLV First-Strand cDNA  Synthesis Kit, separate oligos (MB17302) - Biosciences<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/biosciences.vn\/index.php\/product\/nzy-m-mulv-first-strand-cdna-synthesis-kit-separate-oligos-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NZY M-MuLV First-Strand cDNA  Synthesis Kit, separate oligos (MB17302) - Biosciences\" \/>\n<meta property=\"og:description\" content=\"B\u1ed9 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean NZY M-MuLV, oligo ri\u00eang bi\u1ec7t, l\u00e0 m\u1ed9t h\u1ec7 th\u1ed1ng bao g\u1ed3m t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n c\u1ea7n thi\u1ebft \u0111\u1ec3 t\u1ed5ng h\u1ee3p cDNA s\u1ee3i \u0111\u1ea7u ti\u00ean, ngo\u1ea1i tr\u1eeb RNA khu\u00f4n m\u1eabu. C\u00e1c hexamer ng\u1eabu nhi\u00ean v\u00e0 c\u00e1c \u0111o\u1ea1n m\u1ed3i Oligo(dT)\u00a018\u00a0\u0111\u01b0\u1ee3c cung c\u1ea5p trong c\u00e1c \u1ed1ng ri\u00eang bi\u1ec7t \u0111\u1ec3 thu\u1eadn [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/biosciences.vn\/index.php\/product\/nzy-m-mulv-first-strand-cdna-synthesis-kit-separate-oligos-2\/\" \/>\n<meta property=\"og:site_name\" content=\"Biosciences\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-28T07:55:09+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/biosciences.vn\/wp-content\/uploads\/2024\/10\/NZYtech-.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"700\" \/>\n\t<meta property=\"og:image:height\" content=\"700\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/biosciences.vn\/index.php\/product\/nzy-m-mulv-first-strand-cdna-synthesis-kit-separate-oligos-2\/\",\"url\":\"https:\/\/biosciences.vn\/index.php\/product\/nzy-m-mulv-first-strand-cdna-synthesis-kit-separate-oligos-2\/\",\"name\":\"NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos (MB17302) - 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